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Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.
Alieva, N O; Efremov, A K; Hu, S; Oh, D; Chen, Z; Natarajan, M; Ong, H T; Jégou, A; Romet-Lemonne, G; Groves, J T; Sheetz, M P; Yan, J; Bershadsky, A D.
Afiliação
  • Alieva NO; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Efremov AK; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Hu S; Center for BioImaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117557, Singapore.
  • Oh D; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Chen Z; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Natarajan M; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Ong HT; Department of Chemistry, University of California, Berkeley, CA, 94720, USA.
  • Jégou A; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Romet-Lemonne G; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Groves JT; Institut Jacques Monod, CNRS, Université de Paris, 15 rue Helene Brion, F-75013, Paris, France.
  • Sheetz MP; Institut Jacques Monod, CNRS, Université de Paris, 15 rue Helene Brion, F-75013, Paris, France.
  • Yan J; Mechanobiology Institute, National University of Singapore, T-lab, 5A Engineering Drive 1, Singapore, 117411, Singapore.
  • Bershadsky AD; Department of Chemistry, University of California, Berkeley, CA, 94720, USA.
Nat Commun ; 10(1): 3593, 2019 08 09.
Article em En | MEDLINE | ID: mdl-31399564
ABSTRACT
Filopodia, dynamic membrane protrusions driven by polymerization of an actin filament core, can adhere to the extracellular matrix and experience both external and cell-generated pulling forces. The role of such forces in filopodia adhesion is however insufficiently understood. Here, we study filopodia induced by overexpression of myosin X, typical for cancer cells. The lifetime of such filopodia positively correlates with the presence of myosin IIA filaments at the filopodia bases. Application of pulling forces to the filopodia tips through attached fibronectin-coated laser-trapped beads results in sustained growth of the filopodia. Pharmacological inhibition or knockdown of myosin IIA abolishes the filopodia adhesion to the beads. Formin inhibitor SMIFH2, which causes detachment of actin filaments from formin molecules, produces similar effect. Thus, centripetal force generated by myosin IIA filaments at the base of filopodium and transmitted to the tip through actin core in a formin-dependent fashion is required for filopodia adhesion.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudópodes / Miosinas / Miosina não Muscular Tipo IIA / Forminas / Neoplasias Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudópodes / Miosinas / Miosina não Muscular Tipo IIA / Forminas / Neoplasias Idioma: En Ano de publicação: 2019 Tipo de documento: Article