Alnus sibirica encroachment promotes dissolved organic carbon biodegradation in a boreal peatland.

ABSTRACT

Symbiotic dinitrogen (N2)-fixing trees have been expanding to boreal peatlands, yet its influence on dissolved organic carbon (DOC) biodegradation is unclear. Here, we measured DOC, ammonium­nitrogen (NH4+-N), nitrate­nitrogen (NO3--N), dissolved inorganic nitrogen (DIN), and dissolved total nitrogen (DTN) concentrations, specific ultraviolet absorbance at 254 nm (SUVA254), and humification index in the extracts obtained from peats in the 0-10 cm, 10-20 cm, and 20-40 cm depths in the open peatlands and Alnus sibirica islands in a boreal peatland, Northeast China. Afterwards, the peat extracts were used to assess the effect of N2-fixing woody plant expansion on DOC biodegradation with a 42-day incubation experiment. The expansion of A. sibirica significantly increased NH4+-N, NO3--N, DIN, and DTN concentrations, but did not produce a significant effect on SUVA254 and humification index in the extracts in each depth. Following A. sibirica expansion, DOC biodegradation was enhanced by 24.5%, 15.4%, and 38.3% at 0-10 cm, 10-20 cm, and 20-40 cm depths, respectively. Furthermore, DOC biodegradation was significantly and negatively correlated with DOCDIN and DOCDTN ratios, but exhibited no significant relationship with SUVA254 and humification index. This implied that improved N availability and associated shifts in CN stoichiometry determined the increase in DOC biodegradation following A. sibirica expansion. Our findings suggest that N2-fixing tree encroachment promotes microbial decomposition of DOC through improved N availability in boreal peatlands, which may cause organic C loss from soils in these C-enriched ecosystems.