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Gene Expression Measurement Module (GEMM) for space application: Design and validation.
Peyvan, Kianoosh; Karouia, Fathi; Cooper, John J; Chamberlain, Jeff; Suciu, Dominic; Slota, Michael; Pohorille, Andrew.
Afiliação
  • Peyvan K; Peyvan Systems Inc., Seattle, WA 98109, USA. Electronic address: kia@peyvansystems.com.
  • Karouia F; University of California San Francisco, Department of Pharmaceutical Chemistry, San Francisco, CA 94158, USA; NASA Ames Research Center, Space Biosciences Research Branch, Moffett Field, CA 94035, USA; NASA Ames Research Center, Exobiology Branch, MS 239-4, Moffett Field, CA 94035, USA. Electronic a
  • Cooper JJ; Peyvan Systems Inc., Seattle, WA 98109, USA.
  • Chamberlain J; Peyvan Systems Inc., Seattle, WA 98109, USA.
  • Suciu D; Peyvan Systems Inc., Seattle, WA 98109, USA.
  • Slota M; Peyvan Systems Inc., Seattle, WA 98109, USA.
  • Pohorille A; University of California San Francisco, Department of Pharmaceutical Chemistry, San Francisco, CA 94158, USA; NASA Ames Research Center, Exobiology Branch, MS 239-4, Moffett Field, CA 94035, USA. Electronic address: andrew.pohorille@nasa.gov.
Life Sci Space Res (Amst) ; 22: 55-67, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31421849
In order to facilitate studies on the impact of the space environment on biological systems, we have developed a prototype of GEMM (Gene Expression Measurement Module) - an automated, miniaturized, integrated fluidic system for in-situ measurements of gene expression in microbial samples. The GEMM instrument is capable of (1) lysing bacterial cell walls, (2) extracting and purifying RNA released from cells, (3) hybridizing the RNA to probes attached to a microarray and (4) providing electrochemical readout, all in a microfluidics cartridge. To function on small, uncrewed spacecraft, the conventional, laboratory protocols for both sample preparation and hybridization required significant modifications. Biological validation of the instrument was carried out on Synechococcus elongatus, a photosynthetic cyanobacterium known for its metabolic diversity and resilience to adverse conditions. It was demonstrated that GEMM yielded reliable, reproducible gene expression profiles. GEMM is the only high throughput instrument that can be deployed in near future on space platforms other than the ISS to advance biological research in space. It can also prove useful for numerous terrestrial applications in the field.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Exobiologia / Perfilação da Expressão Gênica Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Exobiologia / Perfilação da Expressão Gênica Idioma: En Ano de publicação: 2019 Tipo de documento: Article