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Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples.
Ichihashi, Yuki; Komatsu, Toru; Kyo, Etsu; Matsuzaki, Hiroyuki; Hata, Keisuke; Watanabe, Toshiaki; Ueno, Tasuku; Hanaoka, Kenjiro; Urano, Yasuteru.
Afiliação
  • Ichihashi Y; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Komatsu T; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Kyo E; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Matsuzaki H; Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Hata K; Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Watanabe T; Department of Surgical Oncology, Graduate School of Medicine , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Ueno T; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Hanaoka K; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
  • Urano Y; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo , Bunkyo-ku , Tokyo 113-0033 , Japan.
Anal Chem ; 91(18): 11497-11501, 2019 09 17.
Article em En | MEDLINE | ID: mdl-31424921
ABSTRACT
We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metaloendopeptidases / Neoplasias Colorretais / Proteoma / Proteômica Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metaloendopeptidases / Neoplasias Colorretais / Proteoma / Proteômica Idioma: En Ano de publicação: 2019 Tipo de documento: Article