Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples.
Anal Chem
; 91(18): 11497-11501, 2019 09 17.
Article
em En
| MEDLINE
| ID: mdl-31424921
ABSTRACT
We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metaloendopeptidases
/
Neoplasias Colorretais
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Proteoma
/
Proteômica
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article