Imidazolium-dysprosium-based magnetic NanoGUMBOS for isolation of hemoglobin.
Talanta
; 205: 120078, 2019 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-31450390
ABSTRACT
A novel imidazolium-dysprosium-based magnetic nanomaterial, i.e. [C16mim]5[Dy(SCN)8] nanoGUMBOS (nanomaterials fabricated from a group of uniform material based on organic salts), was prepared using a facile method for selective hemoglobin (Hb) isolation. In this nanomaterial, the imidazolium cation serves as a selective Hb affinity group, while dysprosium contributes paramagnetic properties. Through a combination of the advantages of ionic liquids, magnetic adsorbent, and nanoscale solid phase extraction, [C16mim]5[Dy(SCN)8] nanoGUMBOS exhibit great selectivity toward Hb and a favorable extraction efficiency of 95.4% when 1â¯mL of 100⯵g/mL Hb solution is processed with 0.6â¯mg of [C16mim]5[Dy(SCN)8] nanoGUMBOS. As the Hb concentration increased to 800⯵g/mL, the adsorption capacity approached â¼840⯵g/mg. The adsorbed protein is recovered with an elution efficiency of 87% by using 1% SDS solution. This novel nanoGUMBOS solid-phase extraction procedure was successfully applied to selective isolation of Hb from human whole blood and verified using SDS-PAGE. This simple strategy is a novel approach towards fabrication and use of a nanoadsorbent for selective isolation of proteins.
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MEDLINE
Assunto principal:
Hemoglobinas
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Nanoestruturas
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Disprósio
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Extração em Fase Sólida
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article