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Electrospun scaffolds limit the regenerative potential of the airway epithelium.
Schwartz, Cynthia M; Stack, Jacob; Hill, Cynthia L; Lallier, Scott W; Chiang, Tendy; Johnson, Jed; Reynolds, Susan D.
Afiliação
  • Schwartz CM; College of Medicine The Ohio State University Columbus Ohio U.S.A.
  • Stack J; Center for Perinatal Research Nationwide Children's Hospital Columbus Ohio U.S.A.
  • Hill CL; Center for Perinatal Research Nationwide Children's Hospital Columbus Ohio U.S.A.
  • Lallier SW; Center for Perinatal Research Nationwide Children's Hospital Columbus Ohio U.S.A.
  • Chiang T; College of Medicine The Ohio State University Columbus Ohio U.S.A.
  • Johnson J; Center for Regenerative Medicine Nationwide Children's Hospital Columbus Ohio U.S.A.
  • Reynolds SD; Department of Otolaryngology Nationwide Children's Hospital Columbus Ohio U.S.A.
Laryngoscope Investig Otolaryngol ; 4(4): 446-454, 2019 Aug.
Article em En | MEDLINE | ID: mdl-31453356
OBJECTIVE: Significant morbidity and mortality are associated with clinical use of synthetic tissue-engineered tracheal grafts (TETG). Our previous work focused on an electrospun polyethylene terephthalate and polyurethane (PET/PU) TETG that was tested in sheep using a long-segment tracheal defect model. We reported that graft stenosis and limited epithelialization contributed to graft failure. The present study determined if the epithelialization defect could be attributed to: 1) postsurgical depletion of native airway basal stem/progenitor cells; 2) an inability of the PET/PU-TETG to support epithelial migration; or 3) compromised basal stem/progenitor cell proliferation within the PET/PU environment. STUDY DESIGN: Experimental. METHODS: Basal stem/progenitor cell frequency in sheep that underwent TETG implantation was determined using the clone-forming cell frequency (CFCF) method. A novel migration model that mimics epithelial migration toward an acellular scaffold was developed and used to compare epithelial migration toward a control polyester scaffold and the PET/PU scaffold. Basal stem/progenitor cell proliferation within the PET/PU scaffold was evaluated using the CFCF assay, doubling-time analysis, and mitotic cell quantification. RESULTS: We report that TETG implantation did not decrease basal stem/progenitor cell frequency. In contrast, we find that epithelial migration toward the PET/PU scaffold was significantly less extensive than migration toward a polyester scaffold and that the PET/PU scaffold did not support basal stem/progenitor cell proliferation. CONCLUSIONS: We conclude that epithelialization of a PET/PU scaffold is compromised by poor migration of native tissue-derived epithelial cells and by a lack of basal stem/progenitor cell proliferation within the scaffold. LEVEL OF EVIDENCE: NA.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article