Collateral vein dynamics in mouse models of venous thrombosis: Pathways consistent with humans.
Thromb Res
; 182: 116-123, 2019 Oct.
Article
em En
| MEDLINE
| ID: mdl-31476485
ABSTRACT
INTRODUCTION:
Prolific collateralization in the venous system has been associated with more severe disease. However, there is a scarcity of information on venogenesis and collateral vessel progression over time. Further, little is understood regarding the relevance of the most common preclinical model-the mouse-for studying venous collateralization. The purpose of this work was to non-invasively and quantitatively characterize collateral vein development and progression in two murine models of deep vein thrombosis using magnetic resonance imaging (MRI).METHODS:
Venous thrombosis (VT) was induced in 12-14-week-old male C57BL/6 mice using either the inferior vena cava (IVC) ligation model (nâ¯=â¯5) or the electrolytic IVC model (nâ¯=â¯5). Magnetic Resonance Imaging (MRI) methods optimized for small venous imaging were used on days 2, 6, 14, and 21 following venous thrombosis induction to quantify collateral development and thrombus volume.RESULTS:
Collateral veins ~150-200⯵m in diameter could be tracked in three dimensions. Collateral pathways were influenced by pre-existing anatomy; mice with bilateral IVC branches showed a predominant superficial collateral pathway (superficial and internal epigastric veins), whereas mice with no lateral branches exhibited a strong intermediate collateral pathway (gonadal and periureteric veins) and were less likely to develop ascending lumbar collaterals. The degree of venogenesis showed a positive correlation with thrombus volume in both models (combined R2â¯=â¯0.64, pâ¯<â¯0.0001).CONCLUSIONS:
Venous collateral pathways in C57BL/6 mice are consistent with those described in humans. Collateral pathways are influenced by pre-existing anatomy, and the degree of collateralization correlates with thrombus volume.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Veias
/
Trombose Venosa
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article