Cutting Edge: Ig H Chains Are Sufficient to Determine Most B Cell Clonal Relationships.
J Immunol
; 203(7): 1687-1692, 2019 10 01.
Article
em En
| MEDLINE
| ID: mdl-31484734
ABSTRACT
B cell clonal expansion is vital for adaptive immunity. High-throughput BCR sequencing enables investigating this process but requires computational inference to identify clonal relationships. This inference usually relies on only the BCR H chain, as most current protocols do not preserve HL chain pairing. The extent to which paired L chains aids inference is unknown. Using human single-cell paired BCR datasets, we assessed the ability of H chain-based clonal clustering to identify clones. Of the expanded clones identified, <20% grouped cells expressing inconsistent L chains. H chains from these misclustered clones contained more distant junction sequences and shared fewer V segment mutations than the accurate clones. This suggests that additional H chain information could be leveraged to refine clonal relationships. Conversely, L chains were insufficient to refine H chain-based clonal clusters. Overall, the BCR H chain alone is sufficient to identify clonal relationships with confidence.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
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Receptores de Antígenos de Linfócitos B
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Rearranjo Gênico de Cadeia Pesada de Linfócito B
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Cadeias Pesadas de Imunoglobulinas
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Bases de Dados Genéticas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article