Novel fast atom bombardment mass spectrometric procedures for glycoprotein analysis.
Biochimie
; 70(11): 1435-44, 1988 Nov.
Article
em En
| MEDLINE
| ID: mdl-3149513
ABSTRACT
We describe procedures, based upon fast atom bombardment mass spectrometry, for characterising the carbohydrate chains in glycoproteins and for determining sites of glycosylation. Strategies for rapidly screening glycoproteins to ascertain the types of sugar chains present and the degree of heterogeneity are presented. Protocols are given for sequencing O- and N-linked glycans. These strategies exploit simple purification steps and afford data at high sensitivity.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Glicoproteínas
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article