Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance.
Biophys Chem
; 254: 106262, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31514114
ABSTRACT
The application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.
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Base de dados:
MEDLINE
Assunto principal:
Ressonância de Plasmônio de Superfície
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Dispositivos Lab-On-A-Chip
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article