Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH).
Methods Mol Biol
; 2032: 323-354, 2019.
Article
em En
| MEDLINE
| ID: mdl-31522427
ABSTRACT
Paroxysmal nocturnal hemoglobinuria (PNH) is a rare but often debilitating disease which may lead to death in up to 35% of patients within 5 years if unrecognized and untreated. Detection of PNH and assessment of PNH clone size in RBC and WBC lineages by flow cytometric analysis has increased in importance due to the availability of novel therapies. These therapies typically block the hemolysis of red blood cells and thus significantly lower the morbidities and mortality associated with this disease. This chapter describes validated, state-of-the-art, high-sensitivity flow cytometric methodologies based on latest published testing guidelines for PNH.
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Base de dados:
MEDLINE
Assunto principal:
Imunofenotipagem
/
Citometria de Fluxo
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Hemoglobinúria Paroxística
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article