Evaluation of a quantitative enzyme-linked immunosorbent assay for feline leukemia virus p27 antigen and comparison to proviral DNA loads by real-time polymerase chain reaction.
Comp Immunol Microbiol Infect Dis
; 67: 101348, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31527012
ABSTRACT
Feline leukemia virus (FeLV) is an oncogenic retrovirus of cats. While higher viral RNA and proviral DNA loads have been correlated with progressive infections and disease, a similar correlation has been suggested for p27 antigen concentrations. This analytical study compared the results of a quantitative ELISA for p27 antigen with quantitative real-time PCR results for FeLV proviral DNA in patient samples. A significant positive correlation between copies of proviral DNA and the concentration of p27 antigen was identified (râ¯=â¯0.761, Pâ¯<â¯0.0001). Samples with high proviral DNA loads, at least 1â¯×â¯106 copies/mL of whole blood, typically had p27 antigen concentrations greater than 30â¯ng/mL in plasma. Samples with proviral DNA loads below this level all had concentrations of p27 antigen in plasma that were less than 10â¯ng/mL. Given this correlation, it is hypothesized that the concentration of p27 antigen at a given point in time may help to indicate the likelihood of a progressive or regressive infection similar to what has been demonstrated for proviral DNA loads.
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MEDLINE
Assunto principal:
DNA Viral
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Ensaio de Imunoadsorção Enzimática
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Vírus da Leucemia Felina
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Antígeno Nuclear de Célula em Proliferação
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Reação em Cadeia da Polimerase em Tempo Real
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article