RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Elife
; 82019 09 19.
Article
em En
| MEDLINE
| ID: mdl-31535974
ABSTRACT
All synapses require fusion-competent vesicles and coordinated Ca2+-secretion coupling for neurotransmission, yet functional and anatomical properties are diverse across different synapse types. We show that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at different central murine synapses and thus contributes to synaptic diversity. At hippocampal pyramidal CA3-CA1 synapses, RIM-BP2 loss has a mild effect on neurotransmitter release, by only regulating Ca2+-secretion coupling. However, at hippocampal mossy fiber synapses, RIM-BP2 has a substantial impact on neurotransmitter release by promoting vesicle docking/priming and vesicular release probability via stabilization of Munc13-1 at the active zone. We suggest that differences in the active zone organization may dictate the role a protein plays in synaptic transmission and that differences in active zone architecture is a major determinant factor in the functional diversity of synapses.
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Base de dados:
MEDLINE
Assunto principal:
Vesículas Sinápticas
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Fibras Musgosas Hipocampais
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Peptídeos e Proteínas de Sinalização Intracelular
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Proteínas do Tecido Nervoso
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Neurônios
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article