Addition of hydrogen peroxide to methylene blue conjugated to ß-cyclodextrin in photodynamic antimicrobial chemotherapy in S. mutans biofilm.
Photodiagnosis Photodyn Ther
; 28: 226-233, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31546042
ABSTRACT
OBJECTIVE:
This study evaluated the effect of hydrogen peroxide addition on ß-cyclodextrin-conjugated methylene blue in antimicrobial photodynamic therapy(a-PDT) in S. mutans biofilm model using laser or light emitting diode (LED) (λâ¯=â¯660â¯nm).METHODS:
A preliminary assay was performed to evaluate the cytotoxicity of hydrogen peroxide in oral fibroblasts by the colorimetric method (MTT). Afterwards, groups were divided into (nâ¯=â¯3, in triplicate) C (negative control), CX - chlorhexidine 0.2% (positive control), P (methylene blue/ß-cyclodextrin), H (Hydrogen Peroxide at 40 µM), PH, L (Laser), LP, LH (Laser+Hydrogen Peroxide), LPH, LED, LEDP, LEDH, and LEDPH. The biofilm was formed in 24â¯h with BHIâ¯+â¯1% sucrose (w/v). Light irradiations were conducted with laser, 9â¯J, 323â¯J/cm2, 113â¯s or with LED, 8.1â¯J, 8.1â¯J/cm2 for 90â¯s. Microbial reduction was evaluated by counting the viable microorganisms of the biofilm after the respective treatments, in a selective culture medium, and laser confocal microscopy evaluation.RESULTS:
LP, LH, LPH, LEDP, LEDH, and LEDPH groups statistically reduced the counts of S.mutans compared with the C group and the log reductions were of 1.87, 1.94, 2.19, 0.91, 0.92, and 1.33, respectively; the addition of hydrogen peroxide did not potentiate the microbial reductions (LPH and LEDPH) compared with the LP and LEDP groups.CONCLUSION:
The association of hydrogen peroxide with the conjugated ß-cyclodextrin nanoparticle as photosensitizer did not result in an enhanced effect of a-PDT; hydrogen peroxide behaved as a photosensitizer, since it reduced the number of S. mutans when associated with laser light.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
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Streptococcus mutans
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Fármacos Fotossensibilizantes
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Biofilmes
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Beta-Ciclodextrinas
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Peróxido de Hidrogênio
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Azul de Metileno
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article