Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin.
PLoS Negl Trop Dis
; 13(9): e0007745, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31560682
ABSTRACT
Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Encefalite Transmitida por Carrapatos
/
Vírus da Encefalite Transmitidos por Carrapatos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article