Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy.
Elife
; 82019 09 30.
Article
em En
| MEDLINE
| ID: mdl-31566566
ABSTRACT
Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO) in mammalian nitric oxide signaling. We determined structures of full-length Manduca sexta sGC in both inactive and active states using cryo-electron microscopy. NO and the sGC-specific stimulator YC-1 induce a 71° rotation of the heme-binding ß H-NOX and PAS domains. Repositioning of the ß H-NOX domain leads to a straightening of the coiled-coil domains, which, in turn, use the motion to move the catalytic domains into an active conformation. YC-1 binds directly between the ß H-NOX domain and the two CC domains. The structural elongation of the particle observed in cryo-EM was corroborated in solution using small angle X-ray scattering (SAXS). These structures delineate the endpoints of the allosteric transition responsible for the major cyclic GMP-dependent physiological effects of NO.
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Base de dados:
MEDLINE
Assunto principal:
Manduca
/
Microscopia Crioeletrônica
/
Guanilil Ciclase Solúvel
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article