Effects of mono- and dialkylglucosides on the characterisation and blood circulation of lipid nanoemulsions.

Aim: Effects of two cosurfactants, n-alkylglycosides with mono- or disaccharide groups - N-nonyl ß-D-glucopyranoside (N-Glu) and N-decyl ß-D-maltoside (D-Mal) - were studied to the stability in saline solution, interaction with serum albumin, and blood circulation of the lipid nanoemulsion (LNE).Methods: The LNEs composed of soybean oil, phosphatidylcholine, and sodium palmitate were prepared without (Control-LNE) and with N-Glu or D-Mal (NG-LNE and DM-LNE, respectively).Results: In saline solution, NG-LNE exhibited a smaller droplet size than Control-LNE, while the size of DM-LNE was significantly increased compared with the other LNEs. The fluorescence resonance energy transfer method showed that the order of albumin interaction was DM-LNE > NG-LNE > Control-LNE. In vivo blood circulation in mice, showed greater fractions of both NG-LNE and DM-LNE remaining in blood over time compared with Control-LNE.Conclusions: The nature of high stability in saline solution and high affinity for serum albumin led to the prolonged circulation of LNE.