A Light-Activated Acyl Carrier Protein "Trap" for Intermediate Capture in Typeâ
II Iterative Polyketide Biocatalysis.
Chemistry
; 25(72): 16515-16518, 2019 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-31596972
ABSTRACT
A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the typeâ
II actinorhodin "minimal" polyketide synthase (PKS), a range of putative ACP-bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT-ICR-MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.
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MEDLINE
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En
Ano de publicação:
2019
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Article