Depletion of dietary aryl hydrocarbon receptor ligands alters microbiota composition and function.
Sci Rep
; 9(1): 14724, 2019 10 11.
Article
em En
| MEDLINE
| ID: mdl-31604984
ABSTRACT
The intestinal microbiota is critical for maintaining homeostasis. Dysbiosis, an imbalance in the microbial community, contributes to the susceptibility of several diseases. Many factors are known to influence gut microbial composition, including diet. We have previously shown that fecal immunoglobulin (Ig) A levels are decreased in mice fed a diet free of aryl hydrocarbon receptor (AhR) ligands. Here, we hypothesize this IgA decrease is secondary to diet-induced dysbiosis. We assigned mice to a conventional diet, an AhR ligand-free diet, or an AhR ligand-free diet supplemented with the dietary AhR ligand indole-3-carbinol (I3C). We observed a global alteration of fecal microbiota upon dietary AhR ligand deprivation. Compared to mice on the conventional diet, family Erysipelotrichaceae was enriched in the feces of mice on the AhR ligand-free diet but returned to normal levels upon dietary supplementation with I3C. Faecalibaculum rodentium, an Erysipelotrichaceae species, depleted its growth media of AhR ligands. Cultured fecal bacteria from mice on the AhR ligand-free diet, but not the other two diets, were able to alter IgA levels in vitro, as was F. rodentium alone. Our data point to the critical role of AhR dietary ligands in shaping the composition and proper functioning of gut microbiota.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Hidrocarboneto Arílico
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Suplementos Nutricionais
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Dieta
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Microbioma Gastrointestinal
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article