Photoaffinity labeling of D-2 dopamine binding subunits from rat striatum, anterior pituitary and olfactory bulb with a new probe, [3H]azidosulpride.
Biochem Biophys Res Commun
; 130(3): 1086-92, 1985 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-3161508
ABSTRACT
A novel photoaffinity probe [3H] azidosulpride has been developed for biochemical studies of D-2 dopamine receptors. This ligand binds to the receptors with high affinity (Kd = 3.1 +/- 0.2 nM) and, upon photoactivation, about 20% of the radioactivity bound to membranes becomes covalently incorporated. More than 90% of this irreversible binding is protectable by dopaminergic agents including D-2 selective compounds, whereas D-1 selective and non-dopaminergic compounds are ineffective. Analysis by sodium dodecylsulfate polyacrylamide gel electrophoresis reveals a single band at Mr = 85 kDa for labeled receptors in striatum, anterior pituitary or olfactory bulb, where pharmacologically distinct binding sites have been previously detected.
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Base de dados:
MEDLINE
Assunto principal:
Bulbo Olfatório
/
Adeno-Hipófise
/
Sulpirida
/
Azidas
/
Marcadores de Afinidade
/
Receptores Dopaminérgicos
/
Corpo Estriado
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article