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Exploration on the Approaches of Diverse Sedimentations in Polyphenol Solutions: An Integrated Chain of Evidence Based on the Physical Phase, Chemical Profile, and Sediment Elements.
Huang, Hao-Zhou; Feng, Bi; Lin, Jun-Zhi; Zhao, Sheng-Yu; Ma, Hong-Yan; Liu, Hai-Yan; Fan, San-Hu; Wu, Zhen-Feng; Xu, Run-Chun; Han, Li; Zhang, Ding-Kun.
Afiliação
  • Huang HZ; Pharmacy College, Chengdu University of TCM, Chengdu, China.
  • Feng B; Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu, China.
  • Lin JZ; Pharmacy College, Chengdu University of TCM, Chengdu, China.
  • Zhao SY; Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu, China.
  • Ma HY; Central Laboratory, Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Liu HY; Pharmacy College, Chengdu University of TCM, Chengdu, China.
  • Fan SH; Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu, China.
  • Wu ZF; Pharmacy College, Chengdu University of TCM, Chengdu, China.
  • Xu RC; Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu, China.
  • Han L; Sanajon Pharmaceutical Group, Chengdu, China.
  • Zhang DK; Sichuan Key Laboratory of Dairy Nutrition and Function, Chengdu, China.
Front Pharmacol ; 10: 1060, 2019.
Article em En | MEDLINE | ID: mdl-31619999
ABSTRACT
Triphala is a famous herbal formula originated in Asia and is popular in America. Due to the high abundance of polyphenols, its oral liquid is unstable and easy to cause precipitate, which results in the loss of activities. However, complex composition and unclear precipitation mechanism hinders the improvement of stability. In this study, the accumulation of precipitation in the storage and its effect on activity were investigated. Then, an integrated chain of evidence was proposed based on the physical phase, chemical profile, and sediment elements. The results showed that antioxidant activity decreased from IC50 115 to 146 µl before and after 90 days of storage, and the anti-fatigue activity decreased from 30.54 to 28.47 min. Turbiscan Lab Expert observed that particle size increased from 106 to 122 nm, and the turbiscan stability index increased from 0 to 14, which indicated that its stability is continuously decreasing. High performance liquid chromatography (HPLC) fingerprint coupled with multivariate statistical analysis identified that these chemical markers changed significantly, such as gallic acid, catechins, and ellagic acid. Loss of catechins tends to be involved in the formation of phlobaphene precipitation. The fact that the new-born ellagic acid in precipitation (0.47 mg/ml) is significantly higher than that reduced in solution (0.25 mg/ml) indicates that it is not only derived from colloid aging. Microscopic observation combined with energy spectrum analysis further confirmed the existence of the multi-precipitates. The crystalline precipitate is ellagic acid, and the other is phlobaphene. In conclusion, based on the evidence chain analysis, this study revealed a systematic change of the whole polyphenol solution system. It provides a novel perspective to understand the sedimentation formation of polyphenol solution, which is an important theoretical contribution to the preparation of polyphenol solutions.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article