Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning.
Methods Mol Biol
; 2070: 157-171, 2020.
Article
em En
| MEDLINE
| ID: mdl-31625095
ABSTRACT
Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations. Here, we describe a method for using a phage-display platform to assess antibody specificity in human serum. We describe the method with reference to the bacteriophage MS2 virus-like particle (VLP) platform, but it can be adapted to other phage-display technologies as well.
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Base de dados:
MEDLINE
Assunto principal:
Levivirus
/
Biblioteca de Peptídeos
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Soro
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Anticorpos de Cadeia Única
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Especificidade de Anticorpos
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Epitopos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article