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Long Non-Coding RNA MEG3 Promotes Apoptosis of Vascular Cells and is Associated with Poor Prognosis in Ischemic Stroke.
Wang, Meiping; Chen, Wenjuan; Geng, Yu; Xu, Chenghua; Tao, Xiaoxiao; Zhang, Yi.
Afiliação
  • Wang M; Department of Neurology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical.
  • Chen W; Department of Neurology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical.
  • Geng Y; Department of Neurology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical.
  • Xu C; Department of Neurology, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University.
  • Tao X; Department of Neurology, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University.
  • Zhang Y; Department of Neurology, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University.
J Atheroscler Thromb ; 27(7): 718-726, 2020 Jul 01.
Article em En | MEDLINE | ID: mdl-31656272
AIM: This study focused on the expression pattern of long non-coding RNA maternally expressed gene 3 (MEG3) and its value in ischemic stroke (IS). METHODS: The expression pattern and the roles of MEG3 in the development of IS were explored in mice IS model and human brain microvascular endothelial cells (hBMECs). A case-control study, including 215 IS patients and 153 controls, was also conducted to investigate its prognostic value. RESULTS: In vivo study showed that MEG3 increased significantly in the IS group (P=0.004), and its level remained stable within 3 to 48h after the onset of IS. Besides, the survival time of the mouse in the high MEG3 group was significantly lower than that in the low MEG3 group (P=0.042). In vitro study showed that oxygen-glucose deprivation (OGD) treatment significantly up-regulated expressions of MEG3, Bax, and cleaved caspase-3, and further promoted apoptosis of hBMECs, while si-MEG3 blocked these effects. A human study showed that MEG3 increased markedly within 48h of IS onset and was positively associated with the National Institutes of Health Stroke Scale (r=0.347, P<0.001), modified Rankin Scale (r=0.385, P<0.001), high-sensitivity C-reactive protein (r=0.221, P=0.002) level, and infarct volume (r=0.201, P=0.006). Overall survival analysis showed that patients with higher MEG3 expression within 48h had a relatively poor prognosis (P<0.001). Meanwhile, multivariate analysis revealed that MEG3 was an independent prognostic marker for unfavorable functional outcome and death in IS patients. CONCLUSIONS: This study suggested that MEG3 might be considered as an intervention point and potential prognostic indicator for IS.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Longo não Codificante / AVC Isquêmico Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Longo não Codificante / AVC Isquêmico Idioma: En Ano de publicação: 2020 Tipo de documento: Article