High clusterin (CLU) mRNA expression levels in tumors of colorectal cancer patients predict a poor prognostic outcome.
Clin Biochem
; 75: 62-69, 2020 Jan.
Article
em En
| MEDLINE
| ID: mdl-31672651
ABSTRACT
OBJECTIVES:
Clusterin (CLU) is a multifunctional intra-/extra-cellular molecular chaperone with indications of serving as a promising prognostic biomarker for colorectal cancer (CRC). Several studies have examined the potential prognostic value of the CLU protein in CRC; however, our research follows an alternative approach, focusing on the CLU mRNA expression. DESIGN ANDMETHODS:
Total RNA from 172 cancerous tissue specimens and 39 paired non-cancerous ones was isolated and 2⯵g of this were subjected to reverse transcription with an oligo-dT primer. The single stranded DNA, which was synthesized, was amplified with an in-house developed highly sensitive and precise qPCR method, using specific pair of primers for the CLU molecule. Finally, an extensive biostatistical analysis took place for the assessment of the results.RESULTS:
Patients with tumors expressing high CLU mRNA levels had a higher probability for poor outcome (relapse and death), comparing to those with CLU mRNA-negative tumors. This association between CLU mRNA expression status and both disease-free survival (DFS) and overall survival (OS) is evident in Cox regression analysis and is also depicted in the Kaplan-Meier survival curves. Consistently, the aforementioned associations and the CLU mRNA expression levels are significantly enhanced as CRC tumors progress from TNM stage I to IV, further supporting the functional implication of CLU in tumorigenesis.CONCLUSIONS:
High CLU mRNA levels in CRC tumors can act as a new adverse prognostic biomarker of DFS and OS for CRC, independent of clinicopathological and biological features of the patient.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Neoplasias Colorretais
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Biomarcadores Tumorais
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Clusterina
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article