Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP.
Nat Commun
; 10(1): 5054, 2019 11 07.
Article
em En
| MEDLINE
| ID: mdl-31699982
ABSTRACT
The mammalian Staufen proteins (Stau1 and Stau2) mediate degradation of mRNA containing complex secondary structures in their 3'-untranslated region (UTR) through a pathway known as Staufen-mediated mRNA decay (SMD). This pathway also involves the RNA helicase UPF1, which is best known for its role in the nonsense-mediated mRNA decay (NMD) pathway. Here we present a biochemical reconstitution of the recruitment and activation of UPF1 in context of the SMD pathway. We demonstrate the involvement of UPF2, a core NMD factor and a known activator of UPF1, in SMD. UPF2 acts as an adaptor between Stau1 and UPF1, stimulates the catalytic activity of UPF1 and plays a central role in the formation of an SMD-competent mRNP. Our study elucidates the molecular mechanisms of SMD and points towards extensive cross-talk between UPF1-mediated mRNA decay pathways in cells.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ribonucleoproteínas
/
RNA Mensageiro
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Transativadores
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Proteínas de Ligação a RNA
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RNA Helicases
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Estabilidade de RNA
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Proteínas do Citoesqueleto
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article