Improved Sensitivity of Intramolecular Strand Displacement Based on Localization of Probes.
Anal Chem
; 91(23): 14808-14811, 2019 12 03.
Article
em En
| MEDLINE
| ID: mdl-31710463
ABSTRACT
Effective intermolecular interaction is required between probe and target molecules for successful detection of biomarkers. Here, we demonstrate that localization of probes on DNA nanostructures improves detection sensitivity and reaction rate. The structural flexibility of DNA nanostructures enabled frequent intramolecular interactions among the localized probes. The Smoluchowski coagulation method and the coarse-grained molecular dynamic software oxDNA were used for theoretical estimation of inter- and intramolecular behaviors of the DNA nanostructures as well as adequate experiments verifying the improvements in sensitivity with probe localization. Remarkably, the probe-localized DNA nanostructure had an increased sensitivity up to 274 times higher than that of the same probes without localization. We believe this achievement represents a wide applicability as a potential design strategy for robust, reliable, and sensitive biosensors.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Técnicas Biossensoriais
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Sondas de DNA
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Nanoestruturas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article