Development and characterization of exendin-4 loaded self-nanoemulsifying system and in vitro evaluation on Caco-2 cell line.
J Microencapsul
; 37(1): 41-51, 2020 Jan.
Article
em En
| MEDLINE
| ID: mdl-31714163
ABSTRACT
Aim:
Aim of this study was to develop exendin-4 and exendin-4/chymostatin loaded self-nanoemulsifying drug delivery system (SNEDDS).Methods:
Surfactants and co-surfactants were mixed, oil phase containing exendin-4 or exendin-4/chymostatin was added dropwise for SNEDDS. Short term physical stability test was performed prior to the release, lipolysis and permeability studies.Results:
SNEDDS containing ethyl oleate Cremophor EL® Labrasol® propylene glycole (1542.521.25 21.25) were selected for in vitro release and intestinal permeability studies for suitable parameters and physical stability test results. SNEDDS were obtained which yielded Grade B nanoemulsions having droplet size below 25 nm. In vitro release studies showed that 73.79% of the peptide was released for 2 h at pH 6.8. Both exendin-4 and exendin-4/chymostatin loaded SNEDDS were non-toxic to Caco-2 cells. Permeability coefficients of both exendin-4 loaded SNEDDS and exendin-4/chymostatin loaded SNEDDS were higher than exendin-4 solution.Conclusions:
Intestinal permeability of exendin-4 has been improved by SNEDDS formulations.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
/
Emulsões
/
Exenatida
/
Hipoglicemiantes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article