Two indoleamines are secreted from rat pineal gland at night and act on melatonin receptors but are not night hormones.
J Pineal Res
; 68(2): e12622, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-31715643
ABSTRACT
INTRODUCTION:
At night, the pineal gland produces the indoleamines, melatonin, N-acetylserotonin (NAS), and N-acetyltryptamine (NAT). Melatonin is accepted as a hormone of night. Could NAS and NAT serve that role too?METHODS:
Concentration-response measurements with overexpressed human melatonin receptors MT1 and MT2 ; mass spectrometry analysis of norepinephrine-stimulated secretions from isolated rat pineal glands; analysis of 24-hour periodic samples of rat blood.RESULTS:
We show that NAT and NAS do activate melatonin receptors MT1 and MT2 , although with lower potency than melatonin, and that in vitro, melatonin and NAS are secreted from stimulated, isolated pineal glands in roughly equimolar amounts, but secretion of NAT was much less. All three were found at roughly equal concentrations in blood during the night. However, during the day, serum melatonin fell to very low values creating a high-amplitude circadian rhythm that was absent after pinealectomy, whereas NAS and NAT showed only small or no circadian variation.CONCLUSION:
Blood levels of NAS and NAT were insufficient to activate peripheral melatonin receptors, and they were invariant, so they could not serve as circulating hormones of night. However, they could instead act in paracrine circadian fashion near the pineal gland or via other higher-affinity receptors.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Glândula Pineal
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Serotonina
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Triptaminas
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Ritmo Circadiano
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Receptor MT1 de Melatonina
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Receptor MT2 de Melatonina
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article