Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor.
Cell J
; 22(2): 133-139, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-31721526
ABSTRACT
Specific developmental characteristics of the chicken make it an attractive model for the generation of transgenic organisms. Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection. Culturing chicken primordial germ cells (PGCs) and their ability for germline transmission ushered in a new stage in this regard. With the advent of CRISPR/Cas9 system, a new phase of studies for manipulating genomes has begun. It is feasible to integrate a desired gene in a predetermined position of the genome using CRISPR/Cas9 system. In this review, we discuss the new approaches and technologies that can be applied to generate a transgenic chicken with regards to recombinant protein productions.
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MEDLINE
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En
Ano de publicação:
2020
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Article