Oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide photolysis with high-power laser irradiation for the treatment of periodontitis.
J Photochem Photobiol B
; 201: 111633, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31726378
ABSTRACT
In the present study, we assessed the oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide (H2O2) photolysis with a 405-nm laser device at an output power of ≥100â¯mW in hamsters. Twenty-four cheek pouches from 12 male Syrian hamsters received 7-min treatment with pure water (PW), 3% H2O2, laser irradiation of PW at 100â¯mW, laser irradiation of 3% H2O2 at 100â¯mW, laser irradiation of PW at 200â¯mW, or laser irradiation of 3% H2O2 at 200â¯mW (nâ¯=â¯4 each). The diameter of the irradiation area was set at 3â¯mm; accordingly, the calculated irradiances (optical power densities) of the 100- and 200-mW laser lights were approximately 1400 and 2800â¯mW/cm2, respectively. In addition, 12 cheek pouches from six animals received laser irradiation of 3% H2O2 at 100â¯mW for 1, 3, or 5â¯min (nâ¯=â¯4 each). Each treatment was repeated three times at 1-h intervals. Macroscopic and histological changes were evaluated 24â¯h after the last treatment. In addition, in vitro bactericidal activity of the treatment against periodontal pathogens was evaluated. We found that 405-nm laser irradiation of 3% H2O2 caused moderate to severe oral mucosal irritation when performed at powers of 100 and 200â¯mW for ≥3â¯min, while the same treatment performed at 100â¯mW for 1â¯min resulted in mild irritation. Moreover, 1-min H2O2 photolysis at 100â¯mW caused aâ¯>4-log decrease in viable bacterial counts. These findings suggest that 1-min H2O2 photolysis, which can effectively kill periodontal pathogens, may be acceptable when a 405-nm laser device is used at 100â¯mW. However, use of the laser at a lower power would be preferable for the prevention of unnecessary oral mucosal irritation.
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Base de dados:
MEDLINE
Assunto principal:
Fotólise
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Peróxido de Hidrogênio
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Lasers
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Anti-Infecciosos
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Mucosa Bucal
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article