Facile fabrication of a high-efficient and biocompatibility biocatalyst for bisphenol A removal.
Int J Biol Macromol
; 150: 948-954, 2020 May 01.
Article
em En
| MEDLINE
| ID: mdl-31730948
ABSTRACT
In this study, we developed a smart microfluidic device to prepare biocatalyst HRP-pCBMA. HRP-pCBMA is composed of horseradish peroxidase (HRP) and zwitterionic polymers poly(carboxybetaine methacrylate) (pCBMA), and could be applied to biodegrade bisphenol A (BPA) efficiently. Compared to free HRP, HRP-pCBMA exhibited an obviously enhanced degrading capability for 1 mM BPA with 99.42% degradation efficiency within 20 min, even being superior to 20-fold amount of free HPR. Besides, HRP-pCBMA displayed high stability against the abrupt changes of environmental factors (temperature, pH and organic solvents), and HRP-pCBMA exhibited a relatively high BPA degradation rate of more than 90% even after 10 cycles. The Kcat and Vmax values of HRP-pCBMA were both 7-fold higher than that of free HRP, indicating significant improvement of the catalytic activity. Furthermore, the cytotoxicity assay indicated HRP-pCBMA has excellent biocompatibility. These results demonstrated that HRP-pCBMA possessed great potential in the bioremediation of BPA.
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MEDLINE
Assunto principal:
Fenóis
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Compostos Benzidrílicos
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Biodegradação Ambiental
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Enzimas
Idioma:
En
Ano de publicação:
2020
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Article