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Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons.
Überbacher, Christa; Obergasteiger, Julia; Volta, Mattia; Venezia, Serena; Müller, Stefan; Pesce, Isabella; Pizzi, Sara; Lamonaca, Giulia; Picard, Anne; Cattelan, Giada; Malpeli, Giorgio; Zoli, Michele; Beccano-Kelly, Dayne; Flynn, Rowan; Wade-Martins, Richard; Pramstaller, Peter P; Hicks, Andrew A; Cowley, Sally A; Corti, Corrado.
Afiliação
  • Überbacher C; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy; Department of Biomedical, Metabolic and Neural Sciences, Università di Modena e Reggio Emilia, Modena, Italy. Electronic address: christa.ueberbacher@i-med.ac.at.
  • Obergasteiger J; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Volta M; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Venezia S; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Müller S; Institute of Human Genetics, Munich University Hospital, Ludwig-Maximilians University, Munich, Germany.
  • Pesce I; CIBIO - Centre for Integrative Biology, Università degli Studi di Trento, Trento, Italy.
  • Pizzi S; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Lamonaca G; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Picard A; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Cattelan G; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Malpeli G; Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, Section of Surgery, University of Verona, Verona, Italy; Department of Diagnostics and Public Health, University of Verona, Verona, Italy.
  • Zoli M; Department of Biomedical, Metabolic and Neural Sciences, Università di Modena e Reggio Emilia, Modena, Italy; Center for Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena, Italy.
  • Beccano-Kelly D; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, University of Oxford, Oxford, UK.
  • Flynn R; James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
  • Wade-Martins R; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, University of Oxford, Oxford, UK.
  • Pramstaller PP; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Hicks AA; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy.
  • Cowley SA; Oxford Parkinson's Disease Centre, University of Oxford, Oxford, UK; James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
  • Corti C; Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy. Electronic address: corrado.corti@eurac.edu.
Stem Cell Res ; 41: 101656, 2019 12.
Article em En | MEDLINE | ID: mdl-31733438
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Transgenes / Proteínas de Fluorescência Verde / Técnicas de Introdução de Genes / Células-Tronco Pluripotentes Induzidas / Neurônios Dopaminérgicos / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Transgenes / Proteínas de Fluorescência Verde / Técnicas de Introdução de Genes / Células-Tronco Pluripotentes Induzidas / Neurônios Dopaminérgicos / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article