Quantitative Jasmonate Profiling Using a High-Throughput UPLC-NanoESI-MS/MS Method.
Methods Mol Biol
; 2085: 169-187, 2020.
Article
em En
| MEDLINE
| ID: mdl-31734925
ABSTRACT
Jasmonic acid (JA) and its many derivatives-collectively referred as jasmonates-occur ubiquitously in land plants and regulate a wide range of stress-responses and development. Measuring these signaling compounds is complicated by the large number of jasmonate derivatives and the comparatively low concentration of these metabolites in plant tissues. We, here, present a selective and sensitive method consisting of a two-phase extraction coupled with liquid chromatography, nanoelectrospray ionization, and mass spectrometry to determine jasmonate levels in tissues and fluids of various plant species. The application of stable deuterium-labelled standards in combination with authentic standards allows the absolute quantification of a multitude of jasmonates and, additionally, the semi-quantitative analysis of further metabolites from the jasmonate pathway.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
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Ciclopentanos
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Espectrometria de Massas por Ionização por Electrospray
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Espectrometria de Massas em Tandem
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Oxilipinas
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Metabolômica
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Ensaios de Triagem em Larga Escala
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article