Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
Int J Biol Macromol
; 147: 1125-1135, 2020 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-31739069
ABSTRACT
A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150. A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa. Thespecific rotation of AGP was detected by a polarimeter and it was +45°. Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose l-arabinose d-xylose d-mannose d-glucose and d-galactose with a molar ratio of 12.332.850.693.051.54. The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of â 6)-ß-d-Glcp-( â backbone and the â4)-α-d-Xylp-(1â, â5)-α-l-Araf-(1â, â3)-α-l-Rhap-(1â, â6)-α-d-Galp-(1â, â3,6)-α-Manp-(1â and â1)-ß-d-Glcp as branches. The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP. The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular. The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure. Furthermore, the AGP exhibited certain inhibition activity on α-glucosidase.
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MEDLINE
Assunto principal:
Polissacarídeos
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Alfa-Glucosidases
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Inibidores de Glicosídeo Hidrolases
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Glycyrrhiza
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article