Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering.

Mougiakos, Ioannis; Orsi, Enrico; Ghiffary, Mohammad Rifqi; Post, Wilbert; de Maria, Alberto; Adiego-Perez, Belén; Kengen, Servé W M; Weusthuis, Ruud A; van der Oost, John

Mougiakos, Ioannis; Orsi, Enrico; Ghiffary, Mohammad Rifqi; Post, Wilbert; de Maria, Alberto; Adiego-Perez, Belén; Kengen, Servé W M; Weusthuis, Ruud A; van der Oost, John.

Afiliação

Mougiakos I; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Orsi E; Bioprocess Engineering, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
Ghiffary MR; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Post W; Bioprocess Engineering, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
de Maria A; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Adiego-Perez B; Bioprocess Engineering, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
Kengen SWM; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Weusthuis RA; Bioprocess Engineering, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
van der Oost J; Systems and Synthetic Metabolism Group, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam, Germany.

Microb Cell Fact ; 18(1): 204, 2019 Nov 25.

Article em En | MEDLINE | ID: mdl-31767004

Assuntos

Sistemas CRISPR-Cas/genética; Edição de Genes/métodos; Engenharia Metabólica/métodos; Rhodobacter sphaeroides/genética; Proteínas de Bactérias/metabolismo; Genoma Bacteriano; Recombinação Homóloga; Hidroxibutiratos/metabolismo; Poliésteres/metabolismo; Rhodobacter sphaeroides/metabolismo

Palavras-chave

Cas9; Genome editing; PHB; Rhodobacter sphaeroides

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rhodobacter sphaeroides / Engenharia Metabólica / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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