Acinetobacter baumannii infections in Amazon Region driven by extensively drug resistant international clones, 2016-2018.
Mem Inst Oswaldo Cruz
; 114: e190232, 2019.
Article
em En
| MEDLINE
| ID: mdl-31778426
ABSTRACT
BACKGROUND:
Acinetobacter baumannii is a leading cause of nosocomial infections. This species is characterised by the presence of pandemic lineages (International Clones) that present a broad antimicrobial resistance profile.OBJECTIVE:
To perform the molecular epidemiology of carbapenem-resistant A. baumannii from a clinical setting in the Amazon Basin, and to characterise their antimicrobial resistance determinants.METHODS:
The genetic relationship of carbapenem-resistant A. baumannii were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Class A, B and D ß-lactamase genes were screened by polymerase chain reaction (PCR) and sequencing. The antimicrobial susceptibility profile was obtained by Disc-diffusion method and minimum inhibitory concentration (MIC) determination.FINDINGS:
All carbapenem-resistant A. baumannii strains belonged to three international clones, IC-1, IC-5 and IC-6, the latter recently reported by the first time in Brazil. The major determinant of carbapenem resistance in IC-1 and IC-5 strains was bla OXA-23, associated with ISAba1 and ISAba3, respectively, while IC-6 harboured the bla OXA-72.CONCLUSIONS:
The A. baumannii epidemiology in Brazilian Amazon Region was unknown. It was demonstrated that A. baumannii XDR international clones were responsible for nosocomial infections in Boa Vista during 2016-2018, revealing that the epidemiological scenario of A. baumannii infections in Amazon Region resembles those from the cosmopolitan regions worldwide.
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Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
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Infecções por Acinetobacter
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Farmacorresistência Bacteriana
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Acinetobacter baumannii
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Antibacterianos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article