Use of whole-genome sequencing in the molecular investigation of care-associated HCoV-OC43 infections in a hematopoietic stem cell transplant unit.
J Clin Virol
; 122: 104206, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31783264
ABSTRACT
BACKGROUND:
While respiratory viral infections are recognized as a frequent cause of illness in hematopoietic stem cell transplantation (HSCT) recipients, HCoV-OC43 infections have rarely been investigated as healthcare-associated infections in this population.OBJECTIVES:
In this report, HCoV-OC43 isolates collected from HSCT patients were retrospectively characterized to identify potential clusters of infection that may stand for a hospital transmission. STUDYDESIGN:
Whole-genome and S gene sequences were obtained from nasal swabs using next-generation sequencing and phylogenetic trees were constructed. Similar identity matrix and determination of the most common ancestor were used to compare clusters of patient's sequences. Amino acids substitutions were analysed.RESULTS:
Genotypes B, E, F and G were identified. Two clusters of patients were defined from chronological data and phylogenetic trees. Analyses of amino acids substitutions of the S protein sequences identified substitutions specific for genotype F strains circulating among European people.CONCLUSIONS:
HCoV-OC43 may be implicated in healthcare-associated infections.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Infecção Hospitalar
/
Genoma Viral
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Infecções por Coronavirus
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Coronavirus Humano OC43
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article