In Situ Detection of MAIT Cells and MR1-Expressing Cells in Tissue Biopsies Utilizing Immunohistochemistry.
Methods Mol Biol
; 2098: 83-94, 2020.
Article
em En
| MEDLINE
| ID: mdl-31792817
ABSTRACT
The mucosa-associated invariant T (MAIT) cells are innate-like T cells that recognize microbial vitamin B2 metabolites presented via MR1, a MHC-I-related protein. MAIT cells are abundant in blood and mucosa, where they display a broad range of functions. Spatial distribution of cells and their proximity to other cells, including infected cells and antigen presenting cells, are crucial components of cell-mediated immunity. Here we describe techniques to detect MAIT cells and MR1-expressing cells in situ, which enable the visualization, distribution, and localization of these cells within their histological context. We provide specific protocols and describe potential advantages and limitations for each of the presented methodologies for studying MAIT cells in human tissues.
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Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
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Antígenos de Histocompatibilidade Classe I
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Antígenos de Histocompatibilidade Menor
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Hibridização In Situ
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Células T Invariantes Associadas à Mucosa
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article