The combination of microfracture with induction of Wnt / ß- Catenin pathway, leads to enhanced cartilage regeneration.
J Orthop Surg Res
; 14(1): 428, 2019 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-31829205
ABSTRACT
INTRODUCTION:
Microfracture does not lead to complete healing of full-thickness cartilage defects. The aim of this study was to evaluate the effect of modifying Wnt/ß-catenin signaling following microfracture, on the restoration of a full-thickness cartilage defect in a rabbit model. The modification of the canonical Wnt pathway was achieved through per os administration of lithium carbonate, which is an intracellular inhibitor of glycogen synthase kinase 3-ß (Gsk3-ß) and therefore induces Wnt/ß-catenin signaling. MATERIALS ANDMETHODS:
Full-thickness cartilage defects of 4 mm in diameter were created in the patellar groove of the right femurs of 18 male New Zealand white rabbits. The rabbits were divided into three groups of six (n = 6) based on post-surgery treatment differences, as follows microfracture only (group 1), microfracture plus lithium carbonate 7 mM in the drinking water for 1 week (group 2), microfracture plus lithium carbonate 7 mM in the drinking water for 4 weeks (group 3). All animals were sacrificed 9 weeks after surgery. The outcome was assessed histologically, by using the International Cartilage Repair Society (ICRS) visual histological scale. Immunohistochemistry for type II collagen was also conducted.RESULTS:
Statistical analysis of the histological ICRS scores showed that group 3 was significantly superior to group 1 in four out of six ICRS categories, while group 2 was superior to 1 in only two out of six.CONCLUSION:
The combination of microfracture and systematic administration of lithium carbonate 7 mM for 4 weeks shows statistically significant superiority in four out of six ICRS categories compared with microfracture only for the treatment of full-thickness cartilage defects in a rabbit experimental model.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Cartilagem
/
Fraturas de Estresse
/
Carbonato de Lítio
/
Beta Catenina
/
Via de Sinalização Wnt
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article