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Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium berghei ANKA Strain in a Murine Model.
Fan, Zhi-Gang; Li, Xiao; Fu, Hai-Yi; Zhou, Li-Min; Gong, Fei-Li; Fang, Min.
Afiliação
  • Fan ZG; Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Li X; Department of Pathogenic Biology, Hainan Medical University, Haikou, 571199, China.
  • Fu HY; Department of Pathogenic Biology, Hainan Medical University, Haikou, 571199, China.
  • Zhou LM; Department of Pathogenic Biology, Hainan Medical University, Haikou, 571199, China.
  • Gong FL; Department of Pathogenic Biology, Hainan Medical University, Haikou, 571199, China.
  • Fang M; Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. flgong@163.com.
Curr Med Sci ; 39(6): 883-889, 2019 Dec.
Article em En | MEDLINE | ID: mdl-31845218
ABSTRACT
Malaria remains a global health problem. The relationship between Plasmodium spp. and the gut microbiota as well as the impact of Plasmodium spp. on the gut microbiota in vertebrate hosts is unclear. The aim of the current study was to evaluate the effect of blood-stage Plasmodium parasites on the gut microbiota of mice. The gut microbiota was analyzed by 16S rRNA sequencing and bioinformatic analyses at three stages. The gut microbiota changed during the three phases the healthy stage, the infection stage, and the cure stage (on the 9th day after malarial elimination). Moreover, the gut microbiota of these infected animals did not recover after malaria infection. There were 254 operational taxonomic units (OTUs) across all three stages, and there were unique strains or OTUs at each stage of the experiment. The percentages of community abundance of 8 OTUs changed significantly (P<0.05). The dominant OTU in both the healthy mice and the mice with malaria was OTU265, while that in the cured mice was OTU234. In addition, the changes in OTU147 were the most noteworthy. Its percentage of community abundance varied greatly, with higher values during malaria than before malaria infection and after malaria elimination. These results indicated that the external environment influenced the gut microbiota after host C57BL/6 mice were infected with blood-stage P. berghei ANKA and that the same was true during and after elimination of blood-stage P. berghei ANKA. In addition, we could not isolate OTU147 for further study. This study identified gut microbiota components that were reconstructed after infection by and elimination of blood-stage P. berghei ANKA in host C57BL/6 mice, and this process was affected by P. berghei ANKA and the external environment of the host.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plasmodium berghei / Bactérias / RNA Ribossômico 16S / Análise de Sequência de DNA / Malária Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plasmodium berghei / Bactérias / RNA Ribossômico 16S / Análise de Sequência de DNA / Malária Idioma: En Ano de publicação: 2019 Tipo de documento: Article