microRNA-21 negatively regulates NF-κB signaling pathway via targeting IL1R1 in miiuy croaker.

ABSTRACT

microRNAs (miR) are non-coding RNAs that regulates many biochemical processes, such as cell growth, proliferation and immune response. In this study, the regulation of microRNA-21 (miR-21) to the nuclear factor kappaB (NF-κB) signaling pathway by target IL1R1 has been researched in miiuy croaker. First, we predicted the target gene of miR-21 through bioinformatics, and found that IL1R1 is a direct target of miR-21. Then, we found that the over-expression of miR-21 mimics and the pre-miR-21 plasmid inhibits the luciferase levels of the wild-type of IL1R1-3'UTR. miR-21 inhibitors increase the luciferase levels of IL1R1-3'UTR. Additionally, we also observed that the miR-21 could negative regulate the IL1R1 at the level of translation. At last, this study will help to further understand the immunomodulatory mechanisms of miRNAs in teleost fish after being invaded by pathogens.