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CD22 and CD72 contribute to the development of scleroderma in a murine model.
Zhao, Chunyan; Matsushita, Takashi; Ha Nguyen, Vinh Thi; Tennichi, Momoko; Fujimoto, Manabu; Takehara, Kazuhiko; Hamaguchi, Yasuhito.
Afiliação
  • Zhao C; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
  • Matsushita T; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
  • Ha Nguyen VT; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
  • Tennichi M; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
  • Fujimoto M; Department of Dermatology, Course of Integrated Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Takehara K; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
  • Hamaguchi Y; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan. Electronic address: yasuhito@med.kanazawa-u.ac.jp.
J Dermatol Sci ; 97(1): 66-76, 2020 Jan.
Article em En | MEDLINE | ID: mdl-31883832
BACKGROUND: Systemic sclerosis (SSc) is a systemic autoimmune disease that is characterized by excessive fibrosis. CD22 and CD72 are B cell-specific cell surface molecules that negatively regulate B cell function. OBJECTIVE: The aim of the present study was to investigate the roles of CD22 and CD72 in a murine scleroderma model. METHODS: The experimental fibrosis model was generated by subcutaneous injection of bleomycin or hypochlorous acid (HOCL) into wild-type (WT), CD22-deficient (CD22-/-), CD72-deficient (CD72-/-) and CD22 and CD72 double-deficient (CD22-/-/CD72-/-) mice. We histologically assessed skin fibrosis and inflammatory cell infiltration. Cytokine and chemokine expression levels were measured by real-time polymerase chain reaction. RESULTS: The severity of fibrosis in the skin and lung was significantly less in CD22-/-, CD72-/-, and CD22-/-/CD72-/- mice than in WT mice in the bleomycin-induced model. In the skin of bleomycin-treated mice, the numbers of CD3+ T cells, CD8+ T cells, and F4/80+ macrophages were significantly lower in CD22-/-, CD72-/-, and CD22-/-/CD72-/- mice than in WT mice. The expression levels of mRNAs for IL-6, TNF-α, TGF-ß, CTGF, IL-1ß, IL-13, CXCL2, and ICAM-1 were significantly lower in CD22-/-, CD72-/-, and CD22-/-/CD72-/- mice than in WT mice. In the HOCL-induced model, both skin and lung fibrosis were ameliorated in CD22-/-, CD72-/- and CD22-/-/CD72-/- mice compared to WT mice. CONCLUSION: These results indicate that CD22 and CD72 likely play crucial roles in skin and lung fibrosis. Moreover, the inhibition of CD22 and CD72 function has potential as a therapeutic approach to SSc.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Pele / Antígenos de Diferenciação de Linfócitos B / Antígenos CD / Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico / Pulmão Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Pele / Antígenos de Diferenciação de Linfócitos B / Antígenos CD / Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico / Pulmão Idioma: En Ano de publicação: 2020 Tipo de documento: Article