[Changes of myocardial function in diabetic rats and its mechanism].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 35(5): 390-392, 2019 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-31894668
ABSTRACT
OBJECTIVE:
To investigate the role of calcium-sensing receptor (CaSR) in the decrease of cardiac function in type 2 diabetic rats.METHODS:
Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups. Rats in the diabetes group were fed with high-glucose and high-fat diet, and intraperitoneal injection of streptozocin (STZ,30 mg/kg) was conducted 4 weeks later to establish a type 2 diabetes model. Cardiac morphological changes were observed by HE staining, cardiac function was detected by echocardiography, and CaSR and PKC-αprotein expressions in cardiac tissue were detected by Western blot.RESULTS:
Compared with the control group, the myocardium of diabetic rats showed irregular contraction zone, decreased expression of CaSR protein, increased expression of PKC-α protein, decreased systolic and diastolic functions, and gradually worsened with the prolongation of the course of the disease.CONCLUSION:
Hyperglycemia inhibits the expression of CaSR protein in myocardium of diabetic rats by activating PKC-α, which can cause intracellular calcium disorder and lead to decreased cardiac function.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
/
Miocárdio
Idioma:
Zh
Ano de publicação:
2019
Tipo de documento:
Article