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The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins.
Suryamohan, Kushal; Krishnankutty, Sajesh P; Guillory, Joseph; Jevit, Matthew; Schröder, Markus S; Wu, Meng; Kuriakose, Boney; Mathew, Oommen K; Perumal, Rajadurai C; Koludarov, Ivan; Goldstein, Leonard D; Senger, Kate; Dixon, Mandumpala Davis; Velayutham, Dinesh; Vargas, Derek; Chaudhuri, Subhra; Muraleedharan, Megha; Goel, Ridhi; Chen, Ying-Jiun J; Ratan, Aakrosh; Liu, Peter; Faherty, Brendan; de la Rosa, Guillermo; Shibata, Hiroki; Baca, Miriam; Sagolla, Meredith; Ziai, James; Wright, Gus A; Vucic, Domagoj; Mohan, Sangeetha; Antony, Aju; Stinson, Jeremy; Kirkpatrick, Donald S; Hannoush, Rami N; Durinck, Steffen; Modrusan, Zora; Stawiski, Eric W; Wiley, Kristen; Raudsepp, Terje; Kini, R Manjunatha; Zachariah, Arun; Seshagiri, Somasekar.
Afiliação
  • Suryamohan K; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Krishnankutty SP; MedGenome Inc., Foster City, CA, USA.
  • Guillory J; AgriGenome Labs Private Ltd, Kochi, India.
  • Jevit M; SciGenom Research Foundation, Bangalore, India.
  • Schröder MS; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Wu M; Molecular Cytogenetics laboratory, Texas A&M University, College Station, TX, USA.
  • Kuriakose B; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Mathew OK; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Perumal RC; AgriGenome Labs Private Ltd, Kochi, India.
  • Koludarov I; AgriGenome Labs Private Ltd, Kochi, India.
  • Goldstein LD; AgriGenome Labs Private Ltd, Kochi, India.
  • Senger K; Ecology and Evolution Unit, Okinawa Institute of Science and Technology, Onna-son, Japan.
  • Dixon MD; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Velayutham D; Department of Bioinformatics and Computational Biology, Genentech, Inc., South San Francisco, CA, USA.
  • Vargas D; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Chaudhuri S; AgriGenome Labs Private Ltd, Kochi, India.
  • Muraleedharan M; AgriGenome Labs Private Ltd, Kochi, India.
  • Goel R; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Chen YJ; MedGenome Inc., Foster City, CA, USA.
  • Ratan A; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Liu P; AgriGenome Labs Private Ltd, Kochi, India.
  • Faherty B; AgriGenome Labs Private Ltd, Kochi, India.
  • de la Rosa G; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Shibata H; Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA.
  • Baca M; Department of Microchemistry Proteomics, and Lipidomics, Genentech, Inc., South San Francisco, CA, USA.
  • Sagolla M; Department of Microchemistry Proteomics, and Lipidomics, Genentech, Inc., South San Francisco, CA, USA.
  • Ziai J; The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
  • Wright GA; Division of Genomics, Medical Institute of Bioregulation, Kyushu University, Fukuouka, Japan.
  • Vucic D; Department of Pathology, Genentech, Inc., South San Francisco, CA, USA.
  • Mohan S; Department of Pathology, Genentech, Inc., South San Francisco, CA, USA.
  • Antony A; Department of Pathology, Genentech, Inc., South San Francisco, CA, USA.
  • Stinson J; College of Veterinary Medicine, Flow Cytometry Shared Resource Laboratory, Texas A&M University, College Station, TX, USA.
  • Kirkpatrick DS; Department of Early Discovery Biochemistry, Genentech, Inc., South San Francisco, CA, USA.
  • Hannoush RN; Department of Molecular Biology, SciGenom Labs, Kochi, India.
  • Durinck S; Department of Molecular Biology, SciGenom Labs, Kochi, India.
  • Modrusan Z; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Stawiski EW; Department of Microchemistry Proteomics, and Lipidomics, Genentech, Inc., South San Francisco, CA, USA.
  • Wiley K; Department of Early Discovery Biochemistry, Genentech, Inc., South San Francisco, CA, USA.
  • Raudsepp T; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Kini RM; Department of Bioinformatics and Computational Biology, Genentech, Inc., South San Francisco, CA, USA.
  • Zachariah A; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
  • Seshagiri S; Molecular Biology Department, Genentech, Inc., South San Francisco, CA, USA.
Nat Genet ; 52(1): 106-117, 2020 01.
Article em En | MEDLINE | ID: mdl-31907489
ABSTRACT
Snakebite envenoming is a serious and neglected tropical disease that kills ~100,000 people annually. High-quality, genome-enabled comprehensive characterization of toxin genes will facilitate development of effective humanized recombinant antivenom. We report a de novo near-chromosomal genome assembly of Naja naja, the Indian cobra, a highly venomous, medically important snake. Our assembly has a scaffold N50 of 223.35 Mb, with 19 scaffolds containing 95% of the genome. Of the 23,248 predicted protein-coding genes, 12,346 venom-gland-expressed genes constitute the 'venom-ome' and this included 139 genes from 33 toxin families. Among the 139 toxin genes were 19 'venom-ome-specific toxins' (VSTs) that showed venom-gland-specific expression, and these probably encode the minimal core venom effector proteins. Synthetic venom reconstituted through recombinant VST expression will aid in the rapid development of safe and effective synthetic antivenom. Additionally, our genome could serve as a reference for snake genomes, support evolutionary studies and enable venom-driven drug discovery.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Genoma / Biologia Computacional / Venenos Elapídicos / Transcriptoma / Naja naja Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Genoma / Biologia Computacional / Venenos Elapídicos / Transcriptoma / Naja naja Idioma: En Ano de publicação: 2020 Tipo de documento: Article