Isolation, structure, and potential biotechnological applications of the exopolysaccharide from Paenibacillus polymyxa 92.
Carbohydr Polym
; 232: 115780, 2020 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-31952589
ABSTRACT
Paenibacillus polymyxa 92, isolated from wheat roots, produced large amounts (38.4â¯g L-1) of exopolysaccharide (EPS) in a liquid nutrient medium containing 10 % (w/v) sucrose. The EPS was precipitated from the culture broth with cold acetone and was purified by gel filtration and anion-exchange chromatography. The molecular mass of the EPS was 2.29-1.10â¯×â¯105 Da. Diffuse reflectance infrared Fourier transform and nuclear magnetic resonance spectra showed that the EPS was a linear ß-(2â6)-linked fructan (levan). Aqueous EPS solutions showed pseudoplastic behavior when shear stress was applied at different temperatures. By using the Ostwald-de Waele model, the rheological characteristics of the EPS solution were ascertained. The sorption capacity of the EPS for Zn(II), Cd(II), Pb(II), and Cu(II) was investigated. Sorption was maximal (qâ¯=â¯481â¯mgâ¯g-1) for Cu(II) ions. In model experiments, treatment of wheat seeds with EPS solution significantly increased the length of seedling roots and shoots.
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MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Biotecnologia
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Paenibacillus polymyxa
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article