Rheostatic Control of Protein Expression Using Tuner Cells.
Biochemistry
; 59(6): 733-735, 2020 02 18.
Article
em En
| MEDLINE
| ID: mdl-31975578
ABSTRACT
We assessed the ability of two strains of Escherichia coli, BL21 (DE3) and Tuner (DE3), to express a variant of the B1 domain of protein G, which forms a side-by-side dimer, by using fluorine-labeling and 19F nuclear magnetic resonance spectroscopy. BL21 cells express the protein in a binary, all-or-none, manner, where more cells express the protein at a high level with an increasing inducer concentration. Tuner cells express the protein in a rheostatic manner, where expression increases across all cells with an increasing inducer concentration.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
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Ressonância Magnética Nuclear Biomolecular
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Proteínas de Escherichia coli
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Proteômica
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article