Protein kinase A accelerates the rate of early stage differentiation of pluripotent stem cells.
Biochem Biophys Res Commun
; 524(1): 57-63, 2020 03 26.
Article
em En
| MEDLINE
| ID: mdl-31980180
ABSTRACT
In normal development, the rate of cell differentiation is tightly controlled and critical for normal development and stem cell differentiation. However, the underlying mechanisms regulating the rate of the differentiation are unknown, and manipulation of the rate of the stem cell differentiation is currently difficult. Here we show that activation of protein kinase A (PKA) accelerates the rate of mouse embryonic stem cell (ESC) differentiation through an early loss of ESC pluripotency markers and early appearance of mesodermal and other germ layer cells. The activation of PKA hastened differentiation by increasing the expression of a histone H3 lysine 9 (H3K9) dimethyltransferase, G9a protein, and the level of a negative epigenetic histone mark, H3K9 dimethylation (H3K9me2), in the promoter regions of the pluripotency markers Nanog and Oct4. These results elucidate a novel role of PKA on ESC differentiation and offer an experimental model for controlling the rate of ESC differentiation.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases Dependentes de AMP Cíclico
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Células-Tronco Pluripotentes
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Células-Tronco Embrionárias Murinas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article