Hydrogen-exchange kinetics studied through analysis of self-decoupling of nuclear magnetic resonance.
J Magn Reson
; 312: 106687, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31982802
ABSTRACT
Hydrogen exchange between solute and water molecules occurs across a wide range of timescales. Rapid hydrogen-exchange processes can effectively diminish 1H-15N scalar couplings. We demonstrate that the self-decoupling of 15N nuclear magnetic resonance can allow quantitative investigations of hydrogen exchange on a micro- to millisecond timescale, which is relatively difficult to analyze with other methods. Using a Liouvillian matrix incorporating hydrogen exchange as a mechanism for scalar relaxation, the hydrogen exchange rate can be determined from 15N NMR line shapes recorded with and without 1H decoupling. Self-decoupling offers a simple approach to analyze the kinetics of hydrogen exchange in a wide range of timescale.
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MEDLINE
Assunto principal:
Espectroscopia de Ressonância Magnética
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Compostos de Amônio
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Hidrogênio
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Isótopos de Nitrogênio
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article