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Tracheal microbial populations in horses with moderate asthma.
Manguin, Estelle; Pépin, Elizabeth; Boivin, Roxane; Leclere, Mathilde.
Afiliação
  • Manguin E; Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Quebec, Canada.
  • Pépin E; Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Quebec, Canada.
  • Boivin R; Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Quebec, Canada.
  • Leclere M; Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Quebec, Canada.
J Vet Intern Med ; 34(2): 986-995, 2020 Mar.
Article em En | MEDLINE | ID: mdl-31985115
ABSTRACT

BACKGROUND:

There are limited data on potential dysbiosis of the airway microbiota in horses with asthma. HYPOTHESIS/

OBJECTIVES:

We hypothesized that the respiratory microbiota of horses with moderate asthma is altered. Our objectives were (a) to quantify tracheal bacterial populations using culture and qPCR, (2) to compare aerobic culture and qPCR, and (c) to correlate bacterial populations with bronchoalveolar lavage fluid (BALF) cytology. ANIMALS Eighteen horses with moderate asthma from a hospital population and 10 controls.

METHODS:

Prospective case-control study. Aerobic culture was performed on tracheal aspirates, and streptococci, Pasteurella multocida, Chlamydophila spp., Mycoplasma spp., as well as 16S (bacterial) and 18S (fungal) rRNA subunits were quantified by qPCR.

RESULTS:

Potential pathogens such as Streptococcus spp., Actinobacillus spp., and Pasteurellaceae were isolated from 8, 5, and 6 horses with asthma and 3, 0, and 2 controls, respectively. There was a positive correlation between Streptococcus spp. DNA and 16S rRNA gene (r ≥ 0.7, P ≤ 0.02 in both groups), but the overall bacterial load (16S) was lower in asthma (1.5 ± 1.3 versus 2.5 ± 0.8 × 104 copy/µL, P < 0.05). There was no association between microbial populations and clinical signs, tracheal mucus or BALF inflammation. CONCLUSIONS AND CLINICAL IMPORTANCE This study does not support that bacterial overgrowth is a common feature of chronic moderate asthma in horses. Lower bacterial load could suggest dysbiosis of the lower airways, either as a consequence of chronic inflammation or previous treatments, or as a perpetuating factor of inflammation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Traqueia / Doenças dos Cavalos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Traqueia / Doenças dos Cavalos Idioma: En Ano de publicação: 2020 Tipo de documento: Article