Detection of Zika Virus Replication in Human Semen by Reverse-Transcription Polymerase Chain Reaction Targeting of Antisense Ribonucleic Acid.
J Infect Dis
; 222(2): 319-323, 2020 06 29.
Article
em En
| MEDLINE
| ID: mdl-32052024
ABSTRACT
BACKGROUND:
Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.METHODS:
We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.RESULTS:
We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values <26) and were obtained within 21 days of symptom onset.CONCLUSIONS:
The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Sêmen
/
Replicação Viral
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RNA Viral
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RNA Antissenso
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Zika virus
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Infecção por Zika virus
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article